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| Registros recuperados: 18 | |
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| Salgado,M.; Aleuy,O. A.; Sevilla,I. A.; Troncoso,E.. |
| ABSTRACTMycobacterium avium subsp. paratuberculosis (Map) is the etiological agent of paratuberculosis. In Chile, information about Map isolation from both domestic ruminant and wildlife species has been accumulating, but it has to be extended to other species. The present study focuses specifically on one wild grazing species, the pudu (Pudu puda), one of the less known South American deer considered an endangered species that shares pastures with cattle in southern Chile, where the greatest part of the country's dairy cattle population is located. Convenient samples from 3 pudus were collected from one dairy farm where Map infection had previously been confirmed in cattle. All three pudus shed the bacterium in feces and the isolates are the same type of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Paratuberculosis; Map; Wildlife; Infection; Spillover. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000501205 |
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| Albuquerque,Pedro Paulo Feitosa de; Santos,André de Souza; Souza Neto,Orestes Luiz de; Kim,Pomy de Cássia Peixoto; Cavalcanti,Erika Fernanda Torres Samico Fernandes; Oliveira,Júnior Mário Baltazar de; Mota,Rinaldo Aparecido; Pinheiro Júnior,José Wilton. |
| Abstract The aim of this study was to detect the IS900 region of Mycobacterium avium subsp. paratuberculosis (MAP) in bovine milk samples using real-time polymerase chain reaction (qPCR) and conventional PCR, and to study the agreement between these tests. A total of 121 bovine milk samples were collected from herds considered positive for MAP, from the State of Pernambuco, Brazil. MAP DNA was detected in 20 samples (16.5%) using conventional PCR and in 34 samples (28.1%) using qPCR. MAP DNA was detected in all of the 6 animal farms studied. Moderate agreement was found between qPCR and conventional PCR results, where the sensitivity and specificity of conventional PCR in relation to qPCR were 50% and 96.6%, respectively. Thus, the IS900 region of MAP was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular biology; Diagnosis; Paratuberculosis; Milk. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100113 |
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| Cho, Jaesung; Tauer, Loren W.; Schukken, Ynte H.; Smith, Rebecca L.; Lu, Zhao; Grohn, Yrjo T.. |
| Infectious diseases play a critical role in determining the profitability of individual farms and maintaining the viability of livestock industries, international trade, and trade policies. Thus, it is critical to analyze the economic consequences of infectious diseases, and the effects of producer strategies to control or eliminate diseases in a cost efficient approach. Also, important is the goal to rally support for the development of public disease control programs. This study examined the long-term feasibility and effectiveness of various producer strategies to prevent and control Johne’s disease in dairy herds, an infectious and incurable disease which has significant economic repercussions for the dairy industry. There are few previous studies... |
| Tipo: Conference Paper or Presentation |
Palavras-chave: Animal disease control; Dairy herd model; Johne's disease; Optimal control model; Paratuberculosis; Agribusiness; Farm Management; Livestock Production/Industries; Research and Development/Tech Change/Emerging Technologies. |
| Ano: 2010 |
URL: http://purl.umn.edu/61318 |
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| Fernández,B; Jolly,A; Colavecchia,S; Fernández,E; Mundo,S.L. |
| La paratuberculosis es una enfermedad granulomatosa crónica intestinal, causada por Mycobacterium avium subespecie paratuberculosis, que afecta a los rumiantes. El objetivo del trabajo fue caracterizar la respuesta inmune humoral sistémica y local de bovinos infectados. Se estudiaron once bovinos, seis infectados (asintomáticos, sintomáticos y terminales) y cinco controles. Se determinaron los niveles de inmunoglobulinas totales y se cuantificaron los subisotipos de IgG específicos a paratuberculosis en suero y materia fecal por ELISA. Los niveles de inmunoglobulinas totales en sueros fueron similares. Sin embargo, se evidenciaron aumentos significativos de IgM e IgG totales, e IgG-específica en materia fecal de los sintomáticos. La IgG1 específica fue... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Paratuberculosis; Bovino; Anticuerpos. |
| Ano: 2011 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982011000100001 |
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| Travería,G.E.; Zumarraga,M.; Etchechoury,I.; Romano,M.I.; Cataldi,A.; Alvarado Pinedo,M.F.; Pavlik,I.; Pribylova,R.; Romero,J.R.. |
| We here identified for the first time the presence of Mycobacterium avium paratuberculosis (MAP) sheep (S) strain in Argentina. IS900 polymerase chain reaction (PCR) was positive. The S strain was compared with MAP cattle (C) strains by using IS1311 PCR-restriction endonuclease analysis (PCR-REA), multiplex PCR and restriction fragment length polymorphism (RFLP) analysis. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Paratuberculosis; Sheep; Typing; PCR; PCR-REA. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300037 |
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| González-Acuña,D.; Neira-Ramirez,V.; Moreno-Salas,L.; Quezada,M.. |
| Descreve-se o primeiro caso de paratuberculose em veado pudu-do-sul (Pudu pudu). O animal foi encontrado na cidade de Concepción, centro sul do Chile, em péssimas condições e com sinais claros de diarréia. Internamente apresentava séria atrofia de gordura no coração, lesões histológicas nos pulmões, fígado, baço e rins, e injúrias intestinais. Diferentes amostras foram preparadas com Ziehl-Neelsen, e os linfonodos mesentéricos presentes no íleo foram fortemente positivos para a bactéria resistente ao ácido. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Southern Pudu; Paratuberculosis; Wildlife; Chile. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000400033 |
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| Marassi,Carla Dray; Fonseca,Leila de Souza; Ristow,Paula; Ferreira,Rachel; Lilenbaum,Walter; Oelemann,Walter M.R.. |
| Paratuberculosis (Johne's disease) is a chronic enteritis in cattle caused by infection with Mycobacterium avium subsp. paratuberculosis (Map). In Brazil, few reports describe the isolation of the organism. The gold standard test for Map is its isolation from tissues or feces. Moreover, bacterial growth is slow and test results are available only after three to five months of incubation Furthermore, shedding of bacilli at levels detectable by fecal culture is irregular and does not occur during the early stages of infection, which compromises the sensitivity of this methodology. The most common immunological tests to identify Map infection are complement fixation test (CFT), agar gel immunodiffusion (AGID) and enzyme-linked immunoassay (ELISA). In Brazil,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Paratuberculosis; Cattle; ELISA; Serology; Diagnosis. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200004 |
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| Lilenbaum,Walter; Ferreira,Rachel; Marassi,Carla Dray; Ristow,Paula; Oelemann,Walter Martins Roland; Fonseca,Leila de Souza. |
| Forty-four cows from five herds infected with tuberculosis (TB) and without paratuberculosis (PTB), and 21 cows from a herd without either infection were studied. The cattle presented concordant results in both the skin test and gamma-interferon assay for TB and two commercial ELISAs for PTB. Animals were divided according to TB test results into Group A with 28 TB-infected animals, Group B with 16 TB-negative animals from infected herds, and Group C with 21 TB-negative cows from a tuberculosis-free herd (which were used as controls). Twenty of 28 animals from Group A (71.4%), 6/16 from Group B (37.5%) and none from Group C were reactive to PTB ELISAs, suggesting that these commercial kits were unable to distinguish between PTB and TB. It is proposed that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Paratuberculosis; Tuberculosis; Mycobacterium bovis; Mycobacterium avium paratuberculosis. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300016 |
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| Magnano,G. G; Macias,A. F; Pérez Zavala,V; Schneider,M. O; Bérgamo,E. G; Giraudo,J. A; Sticotti,E; Mació,M; Bernardelli,A. |
| Actualmente se vincula al Mycobacterium avium subsp. paratuberculosis como potencial agente etiológico implicado en la enfermedad de Crohn en humanos. Una de las vías de ingreso sería a través de la ingestión de leche contaminada. El objetivo fue evaluar la presencia de Map en leche comercial homogeneizada y ultrapasteurizada para consumo humano en supermercados de la ciudad de Río Cuarto, Córdoba, Argentina. Se muestrearon 98 envases de 1 litro de leche entera homogeneizada y ultrapasteurizada de seis marcas comerciales. Previa descontaminación con el método de Cornell modificado, se sembraron en medio de cultivo Herrold con y sin micobactina. Todas las muestras fueron negativas. Como posibles causas de estos resultados se discuten: el origen de la leche... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Paratuberculosis; Micobacterias; Leche; Zoonosis emergente. |
| Ano: 2009 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982009000200003 |
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| Jorge,M.C; Traversa,M.J; Schettino,D.M; Giordano,A; Etchechoury,I; Sanz,H; Romero,C; Grand,H; Paolicchi,F; Romano,M.I. |
| Los camélidos sudamericanos (CS) incluyen cuatro especies, guanaco, vicuña, alpaca y llama (Lama glama). En Argentina las llamas eran consideradas fauna y actualmente ganado, revalorizando su carne, fibra, cueros y pieles, también son un medio de subsistencia. Los CS son susceptibles a las enfermedades ocasionadas por micobacterias. El diagnóstico presuntivo se realiza por los signos clínicos y los hallazgos de necropsia y se confirma por técnicas bacteriológicas, moleculares e histopatología. El objetivo de este trabajo es describir un caso clínico con signos compatibles de paratuberculosis y el diagnóstico de laboratorio en una llama en cautiverio perteneciente a un zoológico de Olavarría, Provincia de Buenos Aires. En la necropsia se observaron lesiones... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enteritis granulomatosa; Lama glama; Mycobacterium avium subespecie avium; Paratuberculosis. |
| Ano: 2008 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982008000100007 |
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| Trangoni,Marcos D.; Gioffré,Andrea K.; Cerón Cucchi,María E.; Caimi,Karina C.; Ruybal,Paula; Zumárraga,Martín J.; Cravero,Silvio L.. |
| In this study, we developed new sets of primers to detect Brucella spp. and M. avium subsp. paratuberculosis (MAP) through isothermal amplification. We selected a previously well-characterized target gene, bscp31, specific for Brucella spp. and IS900 for MAP. The limits of detection using the loop-mediated isothermal amplification (LAMP) protocols described herein were similar to those of conventional PCR targeting the same sequences. Hydroxynaphtol blue and SYBR GreenTM allowed direct naked-eye detection with identical sensitivity as agarose gel electrophoresis. We included the LAMP-based protocol in a rapid identification scheme of the respective pathogens, and all tested isolates were correctly identified within 2 to 3 h. In addition, both protocols... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Loop-mediated isothermal amplification; Molecular typing; Brucellosis; Paratuberculosis. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200619 |
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| Gioffré,Andrea; Correa Muñoz,Magnolia; Alvarado Pinedo,María F.; Vaca,Roberto; Morsella,Claudia; Fiorentino,María Andrea; Paolicchi,Fernando; Ruybal,Paula; Zumárraga,Martín; Travería,Gabriel E.; Romano,María Isabel. |
| Multiple-locus variable number-tandem repeat analysis (MLVA) of Mycobacterium avium subspecies paratuberculosis (MAP) isolates may contribute to the knowledge of strain diversity in Argentina. Although the diversity of MAP has been previously investigated in Argentina using IS900-RFLP, a small number of isolates were employed, and a low discriminative power was reached. The aim of the present study was to test the genetic diversity among MAP isolates using an MLVA approach based on 8 repetitive loci. We studied 97 isolates from cattle, goat and sheep and could describe 7 different patterns: INMV1, INMV2, INMV11, INMV13, INMV16, INMV33 and one incomplete pattern. INMV1 and INMV2 were the most frequent patterns, grouping 76.3% of the isolates. We were also... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Paratuberculosis; Molecular typing; MIRU-VNTR; MLVA. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200557 |
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| Albuquerque,P.P.F.; Cezar,R.D.S.; Pinheiro Junior,J.W.; Grazielle Nascimento,G.; Santos,A.S.; Mota,R.A.. |
| ABSTRACT Paratuberculosis is a chronic and incurable disease that affects ruminants and other domestic animals. It is caused by Mycobacterium avium subsp. paratuberculosis (MAP) that may also be involved in some human diseases such as Crohn's disease, type 1 diabetes, sarcoidosis, multiple sclerosis, and Hashimoto's thyroiditis. The objective of this study was to investigate the occurrence of MAP DNA in samples of artisanal coalho cheese purchased in the State of Pernambuco. Forty samples of coalho cheese submitted to the Real Time Polymerase Chain Reaction (qPCR) technique were analyzed for the detection of the MAP region IS900. 11 (27.5%) were positive with a mean of 195.9 MAP colony forming unit (CFU) per gram of each sample, with a minimum of 30.3... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine; IS900; QPCR; Paratuberculosis. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000601917 |
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| Lilenbaum,W.; Marassi,C.D.; Oelemann,W.M.R.. |
| Paratuberculosis is a chronic enteritis that affects ruminants and is caused by Mycobacterium avium paratuberculosis (Map). The disease is worldwide spread and causes important economic losses. In Brazil, the bacillus was isolated in the south and northeast regions of the country and in Rio de Janeiro, but there are no enough epidemiological studies about its occurrence. Isolation of Map from tissues or fecal samples is 100% specific, but Map shows the most fastidious growth of all mycobacteria. Incubation lasts 8 -12 weeks, with a dependency on exogenous mycobactin J. Diagnostic tests based on specific DNA sequences allow fast and secure identification, and PCR has been used to confirm positive culture results and to identify Map in feces, milk and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Paratuberculosis; Bovine; Mycobacterium; Cattle; Map. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400001 |
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| Paolicchi,F.; Perea,J.; Cseh,S.; Morsella,C.. |
| To study the deficiency of minerals and its relationship with Paratuberculosis, blood, serum, and fecal samples were obtained from 75 adult bovines without clinical symptoms of the disease and from two bovines with clinical symptoms of the disease, from two beef herds with a previous history of Paratuberculosis in the Province of Buenos Aires, Argentina. Serum samples were processed by ELISA and feces were cultured in Herrolds medium. Copper, zinc and iron in serum were quantified by spectrophotometry and selenium was measured by the activity of glutathione peroxidase. We also determined copper, zinc, iron and molybdenum concentrations in pastures and the concentration of sulfate in water. Mycobacterium avium subsp paratuberculosis (Map) was isolated from... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Paratuberculosis; Micronutrients; Selenium; Molybdenum; Copper. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100022 |
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